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Bacillus subtilis early sporulation genes kinA, spo0F, and spo0A are transcribed by the RNA polymerase containing sigma H.

机译：枯草芽孢杆菌早期孢子形成基因kinA，spo0F和spo0A被包含sigma H的RNA聚合酶转录。

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The Bacillus subtilis genes kinA (spoIIJ), spo0F, and spo0A encode components of the sporulation signal transduction pathway. Recent work has suggested that these genes are transcribed by a minor form of RNA polymerase, E sigma H (sigma H is the product of spo0H, another early sporulation gene). We directly tested this hypothesis by performing in vitro transcription assays with reconstituted E sigma H and a set of plasmids containing the kinA, spo0F, and spo0A promoter regions. We were able to obtain distinct transcripts of the expected sizes with all three genes by using linearized or supercoiled templates. Furthermore, primer extension experiments indicate that the transcription start sites for the three genes in vitro and in vivo are the same. In addition, we measured steady-state levels of kinA, spo0F, and spo0A mRNAs during growth in sporulation medium; all of them were increased at or near the beginning of the stationary phase.

机译：枯草芽孢杆菌基因kinA（spoIIJ），spo0F和spo0A编码孢子形成信号转导途径的成分。最近的工作表明，这些基因是通过次要形式的RNA聚合酶E sigma H（sigma H是另一个早期孢子形成基因spo0H的产物）转录的。我们通过用重组的E sigma H和一组含有kinA，spo0F和spo0A启动子区域的质粒进行体外转录测定直接检验了这一假设。通过使用线性化或超螺旋模板，我们能够通过所有三个基因获得预期大小的独特转录本。此外，引物延伸实验表明体外和体内三个基因的转录起始位点是相同的。此外，我们在孢子形成培养基中生长期间测量了kinA，spo0F和spo0A mRNA的稳态水平；所有这些都在固定阶段开始时或附近增加。

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Predich, M; Nair, G; Smith, I;
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年度 1992
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专利

1. Bacillus subtilis early sporulation genes kinA, spo0F, and spo0A are transcribed by the RNA polymerase containing sigma H. [J] . M Predich, G Nair, I Smith Journal of bacteriology . 1992,第9期

机译：枯草芽孢杆菌早期孢子形成基因kinA，spo0F和spo0A被包含sigma H的RNA聚合酶转录。
2. Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis. [J] . M Perego, S P Cole, D Burbulys, Journal of bacteriology . 1989,第11期

机译：磷酸化枯草芽孢杆菌孢子调节蛋白Spo0A和Spo0F的蛋白激酶基因的表征。
3. Expression in Bacillus subtilis of the Bacillus thuringiensis cryIIIA toxin gene is not dependent on a sporulation-specific sigma factor and is increased in a spo0A mutant. [J] . H Agaisse, D Lereclus Journal of bacteriology . 1994,第15期

机译：苏云金芽孢杆菌cryIIIA毒素基因在枯草芽孢杆菌中的表达不依赖于芽孢形成特异性sigma因子，在spo0A突变体中表达增加。
4. Modeling sporulation decisions in Bacillus subtilis as optimal evolutionary decision-making [C] . Morimoto Michael, Arkin Adam, Poolla Kameshwar 2011 American Control Conference . 2011

机译：将枯草芽孢杆菌中的孢子形成决策建模为最佳进化决策
5. Transcriptional regulation of genes encoding spore coat proteins by mother-cell specific sigma(K) RNA polymerase during Bacillus subtilis sporulation. [D] . Ichikawa, Hiroshi Travis. 2000

机译：枯草芽孢杆菌形成过程中母细胞特异性sigma（K）RNA聚合酶对编码孢子外壳蛋白的基因的转录调控。
6. Bacillus subtilis early sporulation genes kinA spo0F and spo0A are transcribed by the RNA polymerase containing sigma H. [O] . M Predich, G Nair, I Smith 1992

机译：枯草芽孢杆菌早期孢子形成基因kinAspo0F和spo0A被包含sigma H的RNA聚合酶转录。
7. Spo0A binds to a promoter used by sigma A RNA polymerase during sporulation in Bacillus subtilis. [O] . Satola, S, Kirchman, P A, Moran, C P 1991

机译：Spo0A与枯草芽孢杆菌形成孢子期间sigma A RNA聚合酶使用的启动子结合。

Bacillus subtilis early sporulation genes kinA, spo0F, and spo0A are transcribed by the RNA polymerase containing sigma H.

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